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KMID : 0368420060490040267
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Journal of Plant Biology
2006 Volume.49 No. 4 p.267 ~ p.270
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Rapidin Vitro propagation and enhanced stevioside accumulation inStevia rebaudiana Bert
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Hwang Sung-Jin
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Abstract
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A very rapid and efficient regeneration method has been established using mature expiants ofStevia rebaudiana Bert. Adventitious shoots were induced from nodal expiants of field-grown plants on four basal media supplemented with various combinations of auxins and cytokinins. The best performance (23.4 ± 2.1 shoots per expiant) was obtained on a Murashige and Skoog (MS) medium containing 2 mg L-1 IAA and 0.5 mg L-1 kinetin. Roots were then produced when thesein vitro- regenerated shoots were transferred to an MS medium supplemented with 3% sucrose and 2 mg L-1 IBA. When acclimatized to soil, the rooted plants had a 98.4% survival rate. Following transplantation in the field, stevioside contents were similar between the regenerated plants (10.68 mg g-1 dry weight) and the mother plants (12.01 mg g-1 dw).
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KEYWORD
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nodal segments, rooting, shoot multiplication, Stevia rebaudiana, stevioside
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